Cell geometry modulates the activation of fibroblasts in 3D tumor microenvironments
Conference talks, 64th Annual Meeting of the Biophysical Society, San Diego, USA
Talk and poster presentation at 64th Annual Meeting of the Biophysical Society
Talks and presentations
Mechano-Genomics: from Cell-Fate Decisions to Biomarkers
Conference talks, Drug Discovery 2019 – Looking Back To The Future, Liverpool, UK
Gave a talk at ELRIG’s :Drug Discovery 2019 – Looking Back To The Future
Multivariate analysis of fibroblast activation in engineered 3D tumor microenvironments
Conference talks, International Conference on Genomes and AI: From Packing to Regulation, Singapore
Talk and poster presentation at International Conference on Genomes and AI: From Packing to Regulation”
Heterogeneity in cell geometric states regulate the selective activation of fibroblasts in engineered 3D tumor microenvironment
Poster presentaton at a conference, Mechanobiology after 10 Years: The Promise of Mechanomedicine, Singapore
Poster presentation at Mechanobiology after 10 Years: The Promise of Mechanomedicine
Authors: Saradha Venkatachalapathy,Shivashankar G.V.
Role of cell geometry and 3D chromatin structure in differential genome regulation
Conference Talk, EMBO Workshop: Nuclear Mechanogenomics, Singapore
Gave a talk at EMBO Workshop: Nuclear Mechanogenomics
Role of 3D chromatin architecture in differential genome regulation
Conference talk, 3rd International Symposium on Mechanobiology, Singapore
Gave a talk at the 3rd International Symposium on Mechanobiology
Nuclear positioning and its translation dynamics is regulated by cell geometry
Poster presentaton at a Conference, Biophysical Society-MBI Thematic Meeting: Mechanobiology of Disease, Singapore
Poster presentation at Biophysical Society-MBI Thematic Meeting: Mechanobiology of Disease
Authors: Radhakrishnan AV,Saradha Venkatachalapathy,Shivashankar G.V.
Abstract
The collective activity of several molecular motors and other active processes generate large forces for directional motion within the cell and a background of fluctuating forces. These processes are vital for a multitude of cellular functions such as migration, division and contraction. In addition, they can also influence the transport and positioning of many cellular organelles by affecting their intracellular dynamics. This creates unique biophysical signatures which are altered in many diseases. In this study, we have used the nucleus as a probe particle to understand the micro-rheological properties of the cytoplasm by using micropatterning techniques to confine cells in two structurally and functionally extreme geometries. We find that nuclear positional dynamics is sensitive to the cytoskeletal organization by studying the effect of actin polymerization, nuclear rigidity, and TNFα cytokine stimulation on the position and diffusive behavior of the nucleus. Taken together, our results suggest that mapping nuclear positional dynamics provides important insights into biophysical properties of the cytoplasm. These biophysical signatures could also be used as an ultrasensitive single-cell assay for early disease diagnostics.